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Title: Utilization of Color from Raspberry (Rubus idaeus L.) for Staining Plant Tissue and Staining Chromosomes
Other Titles: การประยุกต์ใช้สีจากราสเบอร์รี่ (Rubus idaeus L.) สำหรับการย้อมสีเนื้อเยื่อพืชและ โครโมโซมจากปลายรากหอม
Authors: Aiemsaard, Nutthakarn
Rakson, Busada
Chaemchaiyaporn, Teerarat
Keywords: natural dyes
plant tissue
Issue Date: 8-Jul-2021
Publisher: The 13th NPRU National Academic Conference Nakhon Pathom Rajabhat University
Abstract: The objective of this research was to extract dye from the raspberry (Rubus idaeus L.) for use in plant tissue staining and chromosomes to replace the chemical dyes which are expensive and can be environmental pollution. The purpose of these experiments explored the appropriate solvent to extract the frozen raspberry with 4 solvent types including, distilled water, ethanol, methanol and acetic acid with the ratio of 1:1 and 1:3 (plant weight (gram) : quantity of solvent (milliliter)). The results showed that raspberries was extracted with distilled water at 1:1 ratio can clearly stain plant tissue and chromosome staining of mitotic cell division. The average satisfaction values were 4.12 and 4.06, respectively. The raspberries extract were extracted with a 1: 1 ratio distilled water mixed with maltodextrin at the ratio of 100: 30, 100: 35 and 100: 40 (volume (ml): weight (g)), respectively, to maintain the color by freeze drying technique. The extract will be powdered and water soluble. The efficiency of dyes in staining plant tissue and chromosome staining of mitotic cell division were compared. The research result showed that the staining dye obtained from raspberries can stained plant tissue and mitotic cell division, but the color intensity decreased.
Appears in Collections:Proceedings of the 13th NPRU National Academic Conference

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