Utilization of Color from Raspberry (Rubus idaeus L.) for Staining Plant Tissue and Staining Chromosomes

Abstract

The objective of this research was to extract dye from the raspberry (Rubus idaeus L.) for use in plant tissue staining and chromosomes to replace the chemical dyes which are expensive and can be environmental pollution. The purpose of these experiments explored the appropriate solvent to extract the frozen raspberry with 4 solvent types including, distilled water, ethanol, methanol and acetic acid with the ratio of 1:1 and 1:3 (plant weight (gram) : quantity of solvent (milliliter)). The results showed that raspberries was extracted with distilled water at 1:1 ratio can clearly stain plant tissue and chromosome staining of mitotic cell division. The average satisfaction values were 4.12 and 4.06, respectively. The raspberries extract were extracted with a 1: 1 ratio distilled water mixed with maltodextrin at the ratio of 100: 30, 100: 35 and 100: 40 (volume (ml): weight (g)), respectively, to maintain the color by freeze drying technique. The extract will be powdered and water soluble. The efficiency of dyes in staining plant tissue and chromosome staining of mitotic cell division were compared. The research result showed that the staining dye obtained from raspberries can stained plant tissue and mitotic cell division, but the color intensity decreased.