Please use this identifier to cite or link to this item: https://publication.npru.ac.th/jspui/handle/123456789/2125
Title: Screening of pectinase producing Actinomycetes for food-industrial application
Other Titles: การคัดเลือกแอคติโนมัยสีทที่สามารถผลิตเอนไซม์เพคติเนสเพื่อใช้ในอุตสาหกรรมอาหาร
Authors: Sornsanit, Kanya
Saimee, Yuparat
Keywords: Actinomycetes
Enzyme
Pectinase
Food
Issue Date: 13-Aug-2024
Publisher: The 16th NPRU National Academic Conference Nakhon Pathom Rajabhat University
Series/Report no.: Proceedings of the 16th NPRU National Academic Conference;327
Abstract: This research aimed to isolate actinomycetes capable of producing pectinase enzyme from three orchard soils in Sampran district, Nakhon Pathom province, Thailand. The effectiveness of pectin degradation on solid pectin agar plates incubated at room temperature for 5 days was also investigated. Results revealed the successful isolation of 200 actinomycete isolates from the three orchard soils. Upon screening for pectin degradation ability, 81 isolates exhibited clear zones on the solid agar plates, representing 40. 5% of the total isolates tested. The majority of these pectinolytic isolates ( 50 isolates, representing 25% ) exhibited clear zone ratio between 1. 00 and 2. 99 cm. The remaining isolates showed clear zone ratio of 2. 00- 2. 99 cm ( 21 isolates, 10. 5% ) , 3. 00- 3. 99 cm ( 9 isolates, 4. 5% ) , and 0. 1- 0. 99 cm (1 isolate, 0.5%). Notably, 119 isolates (59.5%) failed to produce pectinase. The isolate with the highest ratio of clear area to colony size was isolate SM4, followed by isolate SM4.2, with a clear area ratio of 3.68 and 3.65 cm, respectively. All 81 isolates were tested for pectinase activity. It was found that isolates SM4.2 and SM4 had the highest enzyme activity values of 0. 7932 and 0. 7872 U/ ml, respectively, on the 5th day of cultivation.
URI: https://publication.npru.ac.th/jspui/handle/123456789/2125
ISBN: 978-974-7063-46-2
Appears in Collections:Proceedings of the 16th NPRU National Academic Conference

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