Study on applicable medium in micropropagation of Dendrobium friedericksianum Rchb. f. and Dendrobium crumenatum Sw.

Abstract

The study of suitable formula for propagation of Dendrobium friedericksianum Rchb. f. and D. crumenatum Sw. by tissue culture under the activity production of D. friedericksianum Rchb. f. and D. crumenatum Sw. for commercial medicinal, purposes beginning of October 2018 and ending of September 2021. The objective is to obtain a sterile propagation method for the production of orchids with medicinal potential, consisting of 2 steps: plant induction and proliferation, and root induction and nursery migration. In Induction and proliferation in D. friedericksianum Rchb. f. step, the optimal MS medium combined with 5 mg/L of BA for the maximum number of shoots was 3.4 shoots after culturing for 60 days. While the D. crumenatum Sw. was the VW medium without BA growth regulator, the maximum number of shoots was 6.1 after culturing for 90 days. For the medium of root induction of D. friedericksianum Rchb. f. and D. crumenatum Sw. were MS medium combined with 0.5 mg/L of NAA. The maximum amount of root were 10.4 and 4.0 roots after 90 and 60 days of cultivation, respectively.