Development of Plant Tissue Staining Dye Extracted from Roselle (Hibiscus sabdariffa Linn.)

Abstract

The objective of this research was to select suitable solvents to extract pigments from roselle (Hibiscus sabdariffa Linn.) for use in plant tissue dyeing. with 6 solvent types, 100%methanol, 95% ethanol, distilled water at temperatures 25, 50, 70 and 90 ° C with the ratio of 1:1 (plant weight (gram): quantity of solvent (milliliter)). The results showed that roselle was extracted with distilled water at temperatures 70 °C can clearly stain plant tissue. The average satisfaction value was 4.31. The roselle extract was extracted with a 1:1 ratio of distilled water at temperatures 70 °C mixed with maltodextrin at the ratio of 100: 30 and 100: 40 (volume (ml): weight (g)), respectively, to maintain the color by freeze-drying technique. The extract will be powdered and water soluble. When comparing the dye efficiency in plant tissue dyeing, it was found that it was able to dye the plant tissue well but the dye quality was lighter than the original color.