Effect of Temperature on Total Phenolic Antioxidant Activity and Tyrosinase Inhibition Activity of Elaeocarpus Hygrophilus Kurz

Abstract

This research aimed to investigate the effects of temperature on the total phenolic content, antioxidant activity, and tyrosinase inhibition activity of crude extracts from Elaeocarpus Hygrophilus Kurz leaves. The leaves were dried at three different temperatures 45, 65, and 85 degrees Celsius until they reached a constant weight. The dried samples were then ground and soaked in ethanol solvent for 7 days. After filtering and evaporating the solvent, the crude extracts were analyzed for total phenolic content, total flavonoid content, antioxidant activity, and tyrosinase inhibition activity. The findings showed that the total phenolic content at 45 and 65 degrees Celsius (261.17 and 259 mg GAE/g extract) and the total flavonoid content (89.19 and 85.23 mg QE/g extract) were not significantly different (p≤0.5). When examining antioxidant activity and tyrosinase inhibition activity, it was found that the extracts at 45 degrees Celsius had higher antioxidant activity (IC50 of 11.29 mg/ml) and tyrosinase inhibition activity (IC50 of 16.10 mg/ml) compared to those at 65 and 85 degrees Celsius. These values were also higher than those anti-tyrosinase activity of Trolox and Kojic acid extracts ( IC50 of 14.96 and 20.58 mg/ml, respectively). The results offer valuable baseline data for enhancing the value of Elaeocarpus Hygrophilus Kurz through scientific evidence supporting its properties. This could lead to the development of these extracts as ingredients in cosmetic products.