Research and develop production to increase phytochemical compounds in plant tissue culture of Dendrobium crumenatum Sw. and Dendrobium friedericksianum Rchb. F.

Abstract

Orchids are economically important flowering plants in Thailand. Many species have important herbal medicinal properties. The objective is to obtain a sterile propagation method for the production of D.argumentum Sw. and D. friedericksianum Rchb. F, consisting of 2 steps: plant induction and an increase in the amount of phytochemical compounds, beginning in October 2021 and ending in September 2023. The experiment followed seven treatments, Murashige and Skoog (MS) medium MS medium combined with Jasmonic acid at concentrations of 50, 100, and 150 μM and combined with Yeast Extract at concentrations of 2, 3, and 4 g/ L. for 120 days, The maximum ratio of fresh weight to dry weight of D. crumenatum Sw. and D. friedericksianum Rchb. F. on MS medium was 15. 45 and 14. 19, respectively. Analysis of six phenolic compounds including (2S)-Eriodictyol, (2S)-Homoeriodictyol, Moscatilin, Gigantol, Chrysotoxine and Crepidatin was performed. The results showed that D. crumenatum Sw. was found ( 2S) -Homoeriodictyol, and Chrysotoxine on MS medium combined with Yeast Extract 4 g/ L, were 0. 0145 and 0. 0099 %w/ w, respectively. Crepidatin on the MS medium combined with Jasmonic acid 100 μM, was 0. 0910, %w/ w Gigantol on MS medium combined with 5 mg/ L of N6- Benzyladenine ( BA) was 0.0660 %w/w. Moscatilin in MS medium was 0.0041 %w/w. D. friedericksianum Rchb was found three types of phenolic compounds as follows: Moscatilin and Chrysotoxine in MS medium combined with 2 g/ L of Yeast Extract, were to 0.0051 and 0.0021 %w/w, respectively. Crepidatin on MS medium combined with Jasmonic acid. 50 μM was 0.0053 %w/w.