Utilization of Color from Purple Cabbage (Brassica oleracea var. rubra) for Staining Plant Tissue and Staining Chromosome.

Abstract

The objectives of this research were to select suitable solvents for dye extraction from purple cabbage and to study the efficacy of dye from purple cabbage for staining plant tissue and staining chromosome from onion root tips. Five types of solvents were used: distilled water, 95% methanol, 95% ethanol, 20% acetic acid and 20% hydrochloric acid at a 3:1 ratio (plant weight (g): solvent volume(ml)). The results showed that Extracts from purple cabbage that use a solvent as 20% acetic acid able to staining plant tissue the best, vascular bundle is clearly visible in red and the average satisfaction was equal to 4.32. As for the efficiency of chromosome staining from the tip of the onion root solvent-based paint extracts are 20% hydrochloric acid can give the best chromosome staining effect and the average satisfaction was equal to 3.82.