รายงานการประชุมวิชาการระดับชาติ ครั้งที่ 18 มหาวิทยาลัยราชภัฏนครปฐม https://publication.npru.ac.th/jspui/handle/123456789/2453 2026-10-14T08:13:06Z 2026-10-14T08:13:06Z Chaemchoi, Saran Usuwan, Pornpan Kongsri, Saowanee Karoojee, Supasuta Pompranee, Pitakpong https://publication.npru.ac.th/jspui/handle/123456789/2471 2026-10-14T08:05:45Z 2026-08-28T00:00:00Z

Title: The efficacy of Black Afara Leaves Extracts in Inhibiting the Growth of Ralstonia solanacearum Causing Rhizome Rot in Fingerroot. Authors: Chaemchoi, Saran; Usuwan, Pornpan; Kongsri, Saowanee; Karoojee, Supasuta; Pompranee, Pitakpong Abstract: Fingerroot (Boesenbergia rotunda) is a widely cultivated medicinal plant and culinary spice in Thailand. Farmers in the region often suffer severe losses due to rhizome rot, primarily caused by Ralstonia solanacearum. Traditionally, chemical pesticides have been used to manage thisdisease, but their use results in toxic residues. As a result, alternative, eco-friendly approaches fordisease control have gained attention, including the use of plant extracts with antimicrobialproperties. This study evaluated the efficacy of crude extracts from black afara (Terminalia ivorensis A. Chev) leaves in inhibiting Ralstonia solanacearum, the pathogen responsible forrhizome rot in fingerroot. The antimicrobial activity was assessed using the paper disc diffusionmethod on Tetrazolium Chloride Agar (TZC), testing both fresh and dried black afara leaf extractsat concentrations of 20,000, 40,000, 60,000, 80,000, and 100,000 ppm, with sterilized distilledwater serving as a negative control. The results demonstrated that crude extracts from both freshand dried black afara leaves significantly suppressed the growth of Ralstonia solanacearum (P≤0.05), with higher concentrations leading to greater inhibition. These findings suggest that blackafara leaf extracts offer a promising alternative for managing plant diseases efficiently and reducingreliance on chemical pesticides.

2026-08-28T00:00:00Z Iam-anong, Ronnakorn Usuwan, Pornpan Kongsri, Saowanee Karoojee, Supasuta Pompranee, Pitakpong https://publication.npru.ac.th/jspui/handle/123456789/2470 2026-10-14T08:03:22Z 2026-08-28T00:00:00Z

Title: The Effects of Crude Extracts from Black Afara Leaves on Plant Growth Inhibition Authors: Iam-anong, Ronnakorn; Usuwan, Pornpan; Kongsri, Saowanee; Karoojee, Supasuta; Pompranee, Pitakpong Abstract: Weeds negatively impact agriculture in both production and quality. They compete for water and essential nutrients for plant growth, leading to reduced yields and making harvesting more difficult and time-consuming. Economically, weeds increase production costs, such as labor wages and herbicide expenses. Furthermore, they serve as hosts for diseases and pests. While most farmers rely on chemical herbicides, continuous and improper use can lead to residues in soil, water, air, crops, and the environment. Currently, there are approaches to reducing chemical use by employing naturally active ingredients that are easily biodegradable and safe for users and consumers. Some plants can release secondary metabolites that inhibit the growth of other plants; a phenomenon known as allelopathy. This research studied the inhibitory effects of crude extracts from fresh and dried Terminalia catappa leaves on experimental plants tested on tissue paper-lined petri dishes. At concentrations of 0, 1,000, 5,000, 1,0000, 15,000, 20,000, 25,000, and 30,000 ppm, the inhibitory effect of fresh and dried Terminalia catappa leaf extracts on the growth of monocotyledonous plants (rice) and dicotous plants (Chinese cabbage and eggplant) was significantly different across all concentrations. Increasing the concentration resulted in greater inhibition of seed germination, stem length, and root length in all three experimental plants. Therefore, Terminalia catappa leaf extract has the potential to control plant growth and represents an alternative for reducing the use of chemical herbicides.

2026-08-28T00:00:00Z Srikanlaya, Chonnikarn Wongsil, Siriwan Sabaimuang, Praewa https://publication.npru.ac.th/jspui/handle/123456789/2469 2026-10-14T08:01:03Z 2026-08-28T00:00:00Z

Title: Effect of Hydrocolloids on the Characteristics of Gluten-Free Bread from Coix Seed Flour Authors: Srikanlaya, Chonnikarn; Wongsil, Siriwan; Sabaimuang, Praewa Abstract: The demand for gluten-free bread had increased because of the healthier lifestyle. Some people were intolerant to gluten which led to celiac disease. However, the gluten-free bread typically performed low specific volume, dense and crumbly texture. Hydrocolloids were commonly used as an ingredient to replace gluten, leading to improvement of the gluten-free bread quality. This study aimed to develop gluten-free bread from coix seed flour with the addition of hydrocolloids. The effects of using different hydrocolloid types (Xanthan gum (XG) and Hydroxypropyl methylcellulose (HPMC)) and contents (0.5–2%) on the physical and sensory qualities of gluten-free bread were investigated. Hydrocolloid type and content affected the physical qualities of gluten-free bread. The hardness, specific volume, lightness and whiteness index of gluten-free bread containing XG were higher than those of gluten-free bread containing HPMC. Increasing hydrocolloid contents (XG 0.5–2% and HPMC 0.5–2%) significantly decreased the hardness (p ≤ 0.05). In contrast, the springiness, specific volume, lightness and whiteness index were significantly increased (p ≤ 0.05), whereas the water activity was not significantly different (p > 0.05). In sensory evaluation, the appearance, taste, aroma, flavor, softness, firmness and overall liking scores of all gluten-free breads were not significantly different (p > 0.05). The overall liking scores ranged from 5.70 to 6.14. Additionally, the gluten-free bread containing 1% HPMC was selected as the most appropriate formulation because it received the highest mean overall liking score (6.14) while maintaining desirable physical properties.

2026-08-28T00:00:00Z Wongsupap, Chayapa Kongsorn, Arunee Bincader, Santiti https://publication.npru.ac.th/jspui/handle/123456789/2468 2026-10-14T07:57:40Z 2026-08-28T00:00:00Z

Title: Cellulolytic Microorganisms Selection from Organic Agricultural Soil and Their Ability to Degrade Rice Straw Authors: Wongsupap, Chayapa; Kongsorn, Arunee; Bincader, Santiti Abstract: Rice straw management is a critical issue in agricultural systems due to the loss of soil organic matter, environmental impacts and air pollution, especially Phra Nakhon Si Ayutthaya Province, Thailand, where open-field rice straw burning remains one of the most significant agricultural pollution problems in the country. The use of cellulolytic microorganisms represents a promising approach to accelerate rice straw decomposition and enhance nutrient in soil. This study aimed to investigate the potential of cellulolytic microorganisms isolated from organic agricultural soil and their ability to degrade rice straw. Soil samples were collected from organic fields in Phra Nakhon Si Ayutthaya Province and analyzed for physicochemical properties and total microbial population. Cellulolytic microorganisms were isolated using Congo red assay on CMC agar, and high-efficiency isolates were characterized based on morphological and molecular identification. The selected isolates were evaluated for rice straw degradation under wet laboratory conditions. The results indicated that organic agricultural soil contained diverse microbial populations and more than 42 cellulolytic isolates were obtained. Moreover, isolate SeSt08 (Bacillus paralicheniformis) showed the highest cellulolytic activity with a clear zone diameter of 16.24 mm and showed effective rice straw degradation under laboratory conditions (68.04 – 73.80% weight loss). The potential of cellulolytic microorganisms from organic agricultural soil for development as biological agents for rice straw and agricultural residue management. The results of this study can be applied to reduce open-field burning, increase soil organic matter and support environmentally sustainable agricultural systems in the future.

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